DNA

Part:BBa_K4031003:Design

Designed by: Yuichiro Ikagawa   Group: iGEM21_Gifu   (2021-10-21)


DNA template of crRNA 3 for LbCas12a to detect T7 phage


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Care must be taken when introducing vectors, particularly the type and placement of promoters and terminators. In order to maximize the function of Cas12a as a crRNA, transcription should be performed without any extra sequences being added to the crRNA.


Source

T7 bacteriophage T7p47

References